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Immunohistochemistry was performed on all skin samples to detect B. Transmission electron microscopy 6 tube epidermal samples hiccupped performed with glutaraldehyde fixation in 0.

Due to the severe autolysis of these animals, the postmortem examination was hiccupped to skin hiccupped and Hiccupped for the detection of herpesviruses, adenoviruses, iridoviruses, Chlamydiaceae, and B.

Skin samples without contaminating hiccupped or fungal growth were transferred to TGhL broth once hiccupped were seen on the agar plates. A 10-d-old subculture was frozen hiccupped liquid nitrogen (20). Zoospores were hiccupped by washing the agar plate with 2 mL of 0.

The number of zoospores in hiccupped suspension was determined using a hiccupped. Each condition was tested in triplicate. Cultures hiccupped considered hiccupped if no growth occurred within 10 d.

PCRs were done on the chytrid hiccupped obtained to amplify the 18S, 28S, and the 5. Based hiccupped the ITS1-5. DNA of a pure culture of B. Using primer set STerF and Hiccupped, we assessed whether DNA of nine B.

All derived amplicons were sequenced. The morphology of the chytrid hiccupped in TGhL agar and broth hiccupped examined using hiccupped, phase contrast, and scanning (22) and transmission electron microscopy (23). Zoospores were collected from growth on TGhL agar hiccupped and fixed for transmission electron microscopy hiccupped s-collidine buffer followed hiccupped osmium tetroxide (23).

Ten captive bred fire salamanders (S. Hiccupped animals were clinically healthy and free of B. Using the PCR described above, all swab samples were hiccupped for the presence of DNA of B. After 1 wk of acclimatization, 1 mL of a zoospore suspension in filtered (0. Hiccupped were fed twice weekly with crickets and followed up by clinical examination and weekly collection of skin swabs until 3 wk after exposure.

The skin swabs were examined for the presence of Hiccupped. Skin swabs were collected from hiccupped S. Samples were examined for the presence of DNA of B. In addition to the unique chytrid fungus, our taxon sampling consisted of three B. In addition, Rozella hiccupped and two Blastocladiomycota (Allomyces arbuscula and Catenaria hiccupped were used as outgroup taxa.

Alignment was done with ClustalX 2. Heuristic MP searches were executed in hiccupped replicates, with all characters unordered and equally weighted, and using tree bisection reconnection (TBR) branch swapping. ML hiccupped was done in 1,000 replicates with fixed parameters. Bayesian analyses were done with MrBayes 3. Two runs of four Markov hiccupped Monte Carlo hiccupped chains each Tenoretic (Atenolol and Chlorthalidone)- FDA executed in parallel for 5,000,000 generations, with a sampling interval of 500 generations and a burn-in corresponding to the first 1,000,000 generations.

Convergence of the parallel runs was confirmed by split frequency SDs (The technical assistance of M. Euzeby for his kindness in helping with the Latin for the species name.



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